Abstract: Newcastle disease is common in broiler farms. It causes huge losses to the poultry industry. Because of these, investigating and identifying the disease are important. Moreover, it would be much better to use simpler and cheaper techniques while being more accurate and more sensitive. Therefore, 30 broiler farms, located in Khuzestan province, were sampled and the suspensions were prepared. Then the suspensions were inoculated into 9 to 11 days oldembryonated chicken eggs and after the incubation period were investigated with hemagglutination test. Twelve samples were positive in the haemagglutination test. Finally RT-PCR was performed on the 12 samples to detect Newcastle Disease Virus and the results were positive in six cases. It is important to mention that all six isolates were brain-sourced (also isolated from other tissues) and since all available Newcastle disease vaccines in Iran are lentogen, so the isolates were not from vaccine virus.
Authors: Babak Mohammadian ghalejooghi